Compensatory evolution in NusG improves fitness of drug-resistant M. tuberculosis
Bacterial strains Mtb strains are derivatives of H37Rv unless otherwise noted. ΔbioA Mtb was obtained from the Schnappinger laboratory64. E. coli strains are derivatives of DH5α (NEB), Rosetta2, or BL21(DE3) (Novagen). Mycobacterial cultures Mtb was grown at 37 °C in Difco Middlebrook 7H9 broth or on 7H10 agar supplemented with 0.2% glycerol (7H9) or 0.5% glycerol … Read more